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Galium Aparine Ekstresinin C6-Glioma Hücre Hattında Oluşturulan Glutamat Eksitotoksisitesi Üzerine Etkisinin Araştırılması

Tarih: 22/06/2023 | Yazar: admin

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Investigation of the Effect of Galium Aparine Extract on Glutamate Excitotoxicity Induced in C6-Glioma Cell Line

Yazarlar: Roumina YOUSEFZADE1, Mohaddeseh HASSANPOUR1, Ayşegül ÖZTÜRK2, Sebahattin KARABULUT3

Corresponding author
3Sebahattin KARABULUT, Sivas Cumhuriyet Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu, Tıbbi Hizmetler ve Teknikler Bölümü, Sivas, Türkiye
E-mail: sbkarabulut@cumhuriyet.edu.tr
Other authors
1Sivas Cumhuriyet Üniversitesi Tıp Fakültesi, Dönem 3, Sivas, Türkiye
2Sivas Cumhuriyet Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu, Terapi ve Rehabilitasyon Bölümü, Sivas, Türkiye

ÖZET

Amaç: Beyinin ana uyarıcı nörotransmitteri olan glutamat’ın suprafizyolojik konsantrasyonları glutamat eksitotoksisitesine yol açmaktadır. Galium Aparine (GA) Anadolu’da “yoğurt otu” olarak bilinen ve halk arasında sarılık, kanser ve epilepsi gibi hastalıkların tedavisinde kullanılan bir bitkidir. Bu çalışmada, GA tedavisinin C6 hücrelerinde glutamat nörotoksisitesine karşı hücresel sağ kalıma, oksidan-antioksidan kapasite üzerine etkisi araştırılmıştır.

Yöntem: C6-glioma hücreleri kültüre edildikten sonra; 1.Kontrol grubu, 2.Glutamat grubu, 3. Galium aparine grubu, 4. Galium aparine + Glutamat grubu olmak üzere 4 ayrı grup oluşturuldu. Tüm kontrol ve deney gruplarında uygulanan tedavilerin C6 hücre hattında XTT yöntemiyle nöronal sağ kalıma, Total oksidatif stres (TOS) ve Total antioksidatif stres (TAS) düzeylerine etkileri araştırıldı.

Bulgular: Glutamat’ın hücre sağ kalımını azaltıcı etkisi GA tarafından iyileştirildi. Ayrıca eksitotoksisitenin indüklediği TOS artışı GA tedavisi ile azaltıldı. Buna ilaveten GA hücresel antioksidan düzeyleri artırdı.

Sonuç: Bu sonuçlar aşırı glutamatın yol açtığı hücresel hasarlara karşı GA’nin nöroprotektif etkisine ve bu etkide oksidan-antioksidan dengedeki iyileşmenin yer aldığına işaret etmektedir.

Anahtar kelimeler: C6 glioma, Galium aparine, Glutamat.

ABSTRACT

Aim: Supraphysiological concentrations of glutamate, the brain’s main excitatory neurotransmitter, leads to glutamate excitotoxicity. Galium aparine (GA) is a plant known as a “yogurt herb” in Anatolia and is used in the treatment of diseases such as jaundice, cancer, and epilepsy. This study investigated the effects of GA treatment on cellular viability and oxidant-antioxidant capacity against glutamate neurotoxicity in C6 cells.

Method: C6-glioma cells were cultured and 4 groups were divided into 1. control group, 2. glutamate group, 3. Galium aparine group, 4. Galium aparine + glutamate group. The effects of the treatments administered in all control and experimental groups on neuronal survival, Total oxidative stress (TOS), and Total antioxidative stress (TAS) levels in the C6 cell line were investigated.

Results: The reducing effect of glutamate cytotoxicity on cell survival was ameliorated by GA. In addition, the increase in TOS induced by excitotoxicity was decreased by GA treatment. In addition, GA increased cellular antioxidant levels.

Conclusion: These results indicate that GA has a neuroprotective effect against cellular damage caused by excess glutamate and that the improvement in oxidant-antioxidant balance is involved in this effect.

Keywords: C6 glioma, Galium aparine, Glutamate.

TAM İNDİR Galium Aparine Ekstresinin C6-Glioma Hücre Hattında Oluşturulan Glutamat Eksitotoksisitesi Üzerine Etkisinin Araştırılmasıİndir

How to Cite (APA 7)

Yousefzadeh, R., Hassanpour, M., Öztürk, A., Karabulut, S. (2023). Galium Aparine Ekstresinin C6-Glioma Hücre Hattında Oluşturulan Glutamat Eksitotoksitetesi Üzerine Etkisinin Araştırılması. Health Sciences Student Journal, 3(1), 8-13. https://www.healthssj.com/galium-aparine-ekstresinin-c6-glioma-hucre-hattinda-olusturulan-glutamat-eksitotoksisitesi-uzerine-etkisinin-arastirilmasi/

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Şu konuda daha fazla yazı oku: Research Article veya Volume: 3 Number: 1